Maria Stella Graziani

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Articoli con TAG: glutatione

Effetti della restrizione calorica sullo stress ossidativo nell'obesità: sono miglioramenti transitori?
Effect of caloric restriction on oxidative stress in obesity: are these transient improvements?
<p>Background: the effects of caloric restriction (CR) on oxidative stress in obesity has been previously studied using markers that not always were able to describe all the components of the oxidative-inflammatory picture.<br />Methods: in this study, the redox state of 20 obese was evaluated at baseline and after 30 and 60 days of CR using a complete panel of markers: the majority of them were determined using HPLC methods.<br />Results: before CR (V0), serum peroxides (dROMs) were very high, total antioxidant barrier (BAP) was at the lower limit of the reference interval and C-reactive protein (hsCRP) was increased; on the opposite, glutathione was well within the reference intervals in both total and reduced form. Despite the imbalance of the dROMs/BAP equilibrium, the markers of oxidative damage, such 3-nitrotyrosine (3NT) and 8-hydroxy-deoxyguanosine (8OHdG), index of a mild oxidative-inflammatory imbalance, were not particularly relevant.<br />After 30 days of CR (V30), in addition to the slight improvements of glucose, fructosamine and HOMA-IR, hsCRP was decreased, while BAP and total glutathione were increased, with consequent improvement of the oxidative stress-inflammatory balance (oxidative-inflammation). After 60 days of CR (V60) the improvements observed at V30 appeared to be slowing down for glucose and fructosamine, in slight inverse tendency for HOMA-IR and hsCRP, and decreasing for BAP and glutathione. The slight increase of inter-quartile range (IQR) of 3NT showed a lower counter-regulatory antioxidant response capacity, as if the ameliorative effects of CR in the first period had turned off.<br />Conclusion: the improvements of the oxidative-inflammatory equilibrium appear to be transient in the course of CR. The rearrangements of the gut microbiota during CR and the consequent epigenetic modulations could be responsible for this peculiar trend.</p>
Biochimica Clinica ; 17(1)
Attività fisica e biomarcatori dello stress ossidativo nell’invecchiamento
Physical exercise and oxidative stress biomarkers in the elderly
<p>Introduction: aging is regulated by a number of aging genes including sirtuins, linked to redox status and epigenetically controlled. Physical exercise, depending on intensity, type of training and duration, seems to modulate positively the redox state in aging. It was demonstrated an increase in susceptibility to oxidative stress by the depletion of intracellular glutathione (GSH) and total glutathione (tGSH) levels in the muscles of aged animals. Moreover, physical activity, especially if moderate and regular, seems to activate adaptive mechanisms, reducing body&#39;s vulnerability to oxidative stress and improving systemic metabolic activities.<br />Methods: this study is aimed to evaluate the effects of regular exercise (3 times/week for 2 months) on the levels of radical species (d-ROM test), thiol groups (SHp test), plasma antioxidant capacity (BAPtest), 8-OH-guanosine (COMET test) and some redox-related proteins (HSP27, HSP70, Sirt1 and 2) in 40 healthy elderly subjects, aged from 65 to 74 years. In addition, plasma enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) were evaluated.<br />Results: the enrolled subjects showed oxidative stress before exercise with the presence of radical species, oxidation of purine bases and reduced antioxidant capacity. After the scheduled training, a global improvement of oxidative stress markers was observed with an upregulation of HSP27, HSP70, Sirt1 and Sirt2 expression, as adaptive response.<br />Conclusion: our results underline the importance of these biochemical parameters to monitor oxidative stress in aging and the adaptive redox response to physical exercise in elderly.Therefore, these biomarkers may be routinely used in order to identify individuals at risk for developing oxidative stress related pathologies as well as in personalized physical therapy.</p>
Biochimica Clinica ; 44(1) 036-044
Contributi Scientifici - Scientific Papers