Member area login
You don't have or don't remember the password!
Click Here
Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Andrea di Bello
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282


ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

Articoli con TAG: MGUS

Espressione e valore prognostico del ciclo cellulare e delle aneuploidie nel mieloma multiplo e nella gammopatia monoclonale di significato indeterminato
Expression and prognostic value of cell cycle and aneuploidy in multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS)
<p>In this study, we used multiparameter flow cytometry (MFC) to investigate old and new plasma cell (PC) markers, such as the extrusion pumps of drugs, cluster of differentiation 243 (CD243) and citochemokine receptors (CD197, CD191, CD192, CD196, CD183, CD184, CD126) involved in cellular interactions, locomotion, adhesion and invasion. We studied the different expression of PC in 55 samples of bone marrow of patients affected by MM and MGUS, using MFC and immunomagnetic sorting. The incidence and prognostic significance of the analysis of the cell cycle and aneuploidy were evaluated in order to support the efficacy of MFC. By analysing the immunophenotypic receptor molecules expression and extrusion pumps, we found that CD243, CD192, CD183, CD191 and CD196 showed increased expression in cases of MM, whereas an increased expression of CD197, CD184 and CD126 emerged in MGUS samples. In comparing neoplastic and normal PC in MM samples, we found an increased expression of all receptors on the surface of neoplastic PC. Cell cycle and aneuploidy analysis of all samples showed a pseudodiploid condition with a more difficult prognosis, even considering the old age of patients.</p>
Biochimica Clinica ; 41(3) 228-234
Contributi scientifici - Scientific papers
Gammopatie monoclonali: quadri clinici principali e ruolo del laboratorio
Monoclonal gammopathies: main clinical pictures and role of the clinical laboratory
<p>The finding of a monoclonal gammopathy both by chance and upon clinical suspicion is becoming more and more frequent and requires in-depth clinical, laboratory and instrumental analyses in order to establish the underlying disease, if any. In the last two decades the improvement of the diagnostic tools has led to the identification and precise definition of several clinical entities pathogenetically linked to the monoclonal gammopathy, thus shrinking the field of the &quot;monoclonal gammopathy of undetermined significance&quot;. Such a striking improvement relies on the increasing sensitivity and accuracy of old analyses and availability of new ones (such as the serum Free Light Chain assay). Currently, data from the clinical biochemistry laboratory provide important clues to the diagnosis and prognosis, and are also crucial for monitoring the therapy. These new achievements, along with the availability of new therapeutic options, allowed a significant, sometimes dramatic improvement of the prognosis of many of the gammopathy-related diseases. In this review the main clinical pictures are described along with the contribution of the clinical biochemistry laboratory to the definition of the diagnosis, the risk profile and the monitoring of the specific diseases.</p>
Biochimica Clinica ; 43(4) 366-383
Rassegne - Reviews
Gammopatie monoclonali di significato clinico
Monoclonal gammopathies of clinical significance.
<p>This Opinion is aimed to comment and emphazise the main points raised in a seminal paper published in 2018 in Blood. The paper deals with a number of severe diseases caused by small lymphoid/plasma cell clones. These clones affect different organs by means of the biological properties of the clonal cells themselves and/or of their secretion products such as monoclonal immunoglobulins, cytokines and other molecules. More importantly, the paper also deals with the pathogenetic mechanisms involved in the organ damage and sheds light on most of them. The present comment aims to bring attention to these diseases, but it does not encompass the whole matter. We suggest that the readers involved in this matter refer to the paper in its original version.</p>
Biochimica Clinica ; 43(4) 421-423
Opinioni - Opinions
Inspiegabili alterazioni di esami coagulativi
Unexpected coagulation test abnormalities
<p>A 72 year-old female was screened before a minor orthopaedic surgery with routine coagulation tests, that resulted highly altered. Prothrombin time and activated partial thromboplastin time (PT and aPTT) could not be calculated because the system registered a biphasic curve, protein C and protein S (PC and PS) levels were not measurable and antiphospholipid antibodies were highly positive. Reviewing previous laboratory findings, a serum protein electrophoresis showing the presence of a monoclonal peak in the gamma region was retrieved. A serum immunofixation was then performed, and an IgM kappa monoclonal component was typed; a cryoglobulin test was positive for cryoglobulin type II. To investigate the possible relationship between these laboratory findings, a pool of normal plasma was spiked with the patient IgM monoclonal component at progressively higher concentrations registering a prolongation of PT and aPTT and a slight reduction of PC and PS. The presence of an IgM paraprotein, although not quantitatively relevant, was therefore functionally able to interfere with the coagulation time calculation system. Although coagulation abnormalities in monoclonal gammopathy of undetermined significance are uncommon, these should be attentively investigated, to guarantee a correct laboratory report.</p>
Biochimica Clinica ; 45(1) e7-e10
Casi clinici - Case report