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BC: Articoli scritti da M. Zoppelletto

Evaluation of a multiplex immunochromatographic assay for the rapid detection of carbapenemase-producing Enterobacteriaceaefrom culture colonies
<p>Introduction: the increasing worldwide spread of multidrug resistant bacteria, in particular of carbapenemase-producing Enterobacteriaceae(CPE), represents a serious clinical and public health concern. An accurate and fastdetection of infected patients or colonized carriers is thus mandatory.<br />Aim of this study was to assess the performance of a multiplex immunochromatographic assay (NG-Test CARBA 5,NG Biotech, Guipry, France) for the rapid detection of carbapenemases directly from pure bacterial colonies.<br />Methods: seventy-five non-replicated Enterobacteralesisolates with decreased susceptibility to carbapenems,including 71 Klebsiella pneumoniae, 3Escherichia coli and1Enterobacter cloacae, were analysed with NG-TestCARBA 5. At the same time the combination disk test (CDT) was performed according to the European Committeeon Antimicrobial Susceptibility Testing (EUCAST) indications, while confirmation of carbapenemase production wasachieved by polymerase chain reaction (PCR).<br />Results: PCR assay could find 66 CPE strains, including 64 Klebsiella pneumoniae[53 producing Klebsiellapneumoniaecarbapenemase (KPC), 5 New Delhi metallo-&beta;-lactamase (NDM), 2 class D oxacillinases (OXA-48), 1Verona integron-encoded metallo-&beta;-lactamase (VIM) and 3 co-producing NDM and OXA-48] and 2 Escherichia coli(2 NDM+OXA-48) while 9 isolates were found as non-carbapenemase producing: 7 Klebsiella pneumoniae, 1Escherichia coli, 1Enterobacter cloacae. CDT allowed us to consider those 9 strains as extended spectrum &beta;-lactamase (ESBL) or AmpC &beta;-lactamase producers. NG-Test CARBA 5 successfully identified 66/66 CPE showing100% sensitivity and 100% specificity. Unlike NG-Test CARBA 5, CDT was not able to correctly identify 5 strains co-producing NDM and OXA-48 carbapenemases.<br />Conclusion: NG-Test CARBA 5 is a reliable assay that can be useful in settings requiring a rapid identification of CPEdirectly from culture colonies. Moreover, this test is an easy-to-use option that could avoid misidentification ofcarbapenemases co-producers strains.</p>
Biochimica Clinica ; 17(1)
Contributi Scientifici - Scientific Papers