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BC: Articoli scritti da M. Visconti

Valutazione molecolare nella leucemia mieloide cronica
Molecular monitoring in chronic myeloid leukemia (CML)
<p>The pathognomonic genetic alteration in CML is the&nbsp;formation of the BCR-ABL fusion gene, which produces a constitutively active tyrosine kinase that drives leukemic&nbsp;transformation. Targeted tyrosine kinase inhibitor treatment is the cornerstone of modern therapy for this hematologic&nbsp;malignancy. Analysis of BCR-ABL [through reverse transcriptase-quantitative polymerase chain reaction (RT-QPCR)]&nbsp;is the gold standard approach for quantitatively assessing minimal residual disease and monitoring the efficacy of&nbsp;tyrosine kinase inhibitor therapy in CML patients. The continuous therapeutic improvement has led to increasingly&nbsp;ambitious treatment endpoints, which, in turn, require more and more refined measurement and definition of&nbsp;molecular response levels. For these reasons standardization efforts of monitoring by RT-QPCR are now focused on&nbsp;ensuring reliable and harmonized expression of quantitative results.</p>
Biochimica Clinica ; 39(2) 094-099
Rassegne - Reviews
 
Flow citometry for screening acute urinary tract infections and differentiation between Gram positive and Gram negative bacteria
<p>Urine culture is the most frequent test in microbiology laboratories. A screening tool, providing fast and reliable results&nbsp;to rule-out urinary tract infection (UTI), would be of great importance. We studied 1043 consecutive urine samples by&nbsp;Sysmex UF-1000i analyzer. Comparison was made by robotic urine culture on chromogenic agar with 1 &mu;L loop,&nbsp;using 10<sup>5</sup> CFU/mL as a limit of positive growth. We evaluated bacteria quantification for rapid exclusion of UTI and&nbsp;bacteria forward scatter (B_FSC) in preliminary discrimination of UTI caused by Gram positive or Gram negative&nbsp;bacteria. For exclusion of UTI, the best cut-off value was 130 bacteria/&mu;L. At this threshold, the sensitivity (SE) was&nbsp;0.98 and the specificity (SP) 0.75. For exclusion of UTI sustained by Gram positive bacteria, the best cut-off value for&nbsp;B_FSC was 25ch. At this threshold, SE was 0.68 and SP was 0.89.</p>
Biochimica Clinica ; 38(6) 625-629
Contributi scientifici - Scientific Papers