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BC: Articoli scritti da G. Saccani

Esame fisico, chimico e morfologico delle urine: proposta di linee guida per la fase analitica del Gruppo Intersocietario Analisi delle Urine (GIAU)
Physical, chemical and morphological urine examination: proposed guidelines for the analytical phase by the Intersociety Urinalysis Group
<p>With these guidelines, the Intersociety Urinalysis Group (GIAU) aims to stimulate the following aspects: a) improvement and standardization of the analytical approach to physical, chemical and morphological urine examination (ECMU); b) to emphasize the value added to ECMU by automated analyzers for the study of the morphology of the corpuscular fraction urine; c) improvement of the chemical analysis of urine with particular regard to the reconsideration of the diagnostic significance of parameters that are traditionally evaluated in dipstick analysis, together with an increasing awareness of the limits of sensitivity and specificity of this analytical method; d) to increase the awareness of the importance of professional skills in the field of urinary morphology and of the relationships with clinicians; e) implementation of a policy for the evaluation of the analytical quality by using, in addition to traditional IQC and EQA, a program for the evaluation of morphological competence; f) to stimulate the diagnostic industry to focus research efforts and development methodology and instrumental catering to the needs of clinical diagnosis. The hope is to revalue the enormous diagnostic potential of ECMU, by implementing an urinalysis based on personalized diagnostic needs.</p>
Biochimica Clinica ; 40(4) 353-382
Documenti SIBioC - SIBioC Documents
 
Flow citometry for screening acute urinary tract infections and differentiation between Gram positive and Gram negative bacteria
<p>Urine culture is the most frequent test in microbiology laboratories. A screening tool, providing fast and reliable results&nbsp;to rule-out urinary tract infection (UTI), would be of great importance. We studied 1043 consecutive urine samples by&nbsp;Sysmex UF-1000i analyzer. Comparison was made by robotic urine culture on chromogenic agar with 1 &mu;L loop,&nbsp;using 10<sup>5</sup> CFU/mL as a limit of positive growth. We evaluated bacteria quantification for rapid exclusion of UTI and&nbsp;bacteria forward scatter (B_FSC) in preliminary discrimination of UTI caused by Gram positive or Gram negative&nbsp;bacteria. For exclusion of UTI, the best cut-off value was 130 bacteria/&mu;L. At this threshold, the sensitivity (SE) was&nbsp;0.98 and the specificity (SP) 0.75. For exclusion of UTI sustained by Gram positive bacteria, the best cut-off value for&nbsp;B_FSC was 25ch. At this threshold, SE was 0.68 and SP was 0.89.</p>
Biochimica Clinica ; 38(6) 625-629
Contributi scientifici - Scientific Papers