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BC: Articoli scritti da M. Marini

Valutazione delle caratteristiche analitiche di un nuovo strumento per l’elettroforesi capillare delle proteine sieriche
Evaluation of the analytical performance of a new capillary electrophoresis system for serum protein analysis
<p>Electrophoresis techniques are used in clinical laboratories to detect, type and quantify monoclonal&nbsp;components (MC), and are therefore of pivotal importance in the management of patients with monoclonal&nbsp;gammopathies. Capillary electrophoresis (CE) is an attractive alternative to agarose gel electrophoresis (AGE)&nbsp;because it offers a higher degree of automation without requiring the use of toxic reagents. Aim of this study was to&nbsp;evaluate analytical characteristics of a recently marketed instrument for CE, comparing it with both a well established&nbsp;CE and with AGE. The new system (V8, Helena Biosciences) was compared for MC detection with Capillarys (Sebia)&nbsp;and for typing with both the AGE Hydrasys system (Sebia) and the immunosubtraction technique on Capillarys. All&nbsp;the evaluated analytical characteristics were related to MC investigations, i.e. detection, typing and quantification. The&nbsp;interference effect of some substances and the carryover were also investigated. For MC detection, an agreement of&nbsp;98.5% has been demonstrated between the two CE systems; the discordant samples show a MC &lt;1 g/L. The limit of&nbsp;detection for a MC was 0.5 g/L. A total agreement in the typing of MC &gt;5 g/L between the two CE systems was&nbsp;observed. V8 was able to correctly characterize 18 MC with concentration &lt;5 g/L out of 20 samples typed by AGE&nbsp;immunofixation. V8 can quantify MC with elevated precision (CV 3,5% and 1,2% for <span style="font-family:symbol">b</span>- and <span style="font-family:symbol">g</span>-zone MC, respectively)&nbsp;and the measurement is linear till &lt;1 g/L. Fibrinogen and contrast media appear in the CE patterns as homogeneous&nbsp;bands MC like. From these results, we conclude that the V8 analytical characteristics are fully adequate for the&nbsp;system use in clinical laboratories for managing patients with monoclonal gammopathies.</p>
Biochimica Clinica ; 38(4) 295-301
Contributi scientifici - Scientific Papers
 
Un caso di macrolattatodeidrogenasemia
A case of macrolactatedehydrogenasemia
<p>We present here the detection of a lactate dehydrogenase (LDH) macroenzyme in the blood of an apparently healthy female. The finding of an increased LDH activity during routine biochemical investigations led to a number of further laboratory and imaging examinations. When the diagnostic problem was finally posed to the laboratory consultant, a serum treatment with polyethylene glycol and an isoenzyme electrophoretic separation allowed to demonstrate the presence of a circulating macroenzyme. Macroenzymes have been described from almost 50 years, but despite many efforts it was difficult to recognize a pathogenetic role. Their recognition is, however, important in order to avoid expensive and cumbersome procedures to explain elevated enzyme values.</p>
Biochimica Clinica ; 36(1) 66-68
Casi Clinici - Case Report