Maria Stella Graziani

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Martina Zaninotto

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Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

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BC: Articoli scritti da G. Gambaro

Utilità della spettroscopia all’infrarosso per l’analisi dei calcoli urinari
Usefulness of infrared spectroscopy for stone analysis: a case study
<p>A 22-year-old male with recurrent bilateral stone disease and positive family history for stones was referred in March 2015 to the Metabolic Stone Clinic of the Policlinico Gemelli Hospital; a metabolic assessment revealed a number of abnormalities including hypercalciuria, hypocitraturia and abnormally high fasting urine pH. The previously expelled urinary calculus was sent to the clinical laboratory for the biochemical analysis by the infrared method. Fourier transform infrared spectroscopy (FT-IR) analysis identifying brushite and carbapatite, together with the finding of high urine pH, raised the suspicion of distal renal tubular acidosis which was subsequently confirmed by the urinary acidification test. The semiquantitative method of stone composition analysis would have yielded a mixture of calcium, ammonium, phosphate and magnesium, thus likely directing the diagnostic work-up, together with the abnormally high urine pH, towards infectious stones (e.g., by urease-producing bacteria). The information obtained by FT-IR analysis allowed clinicians to correctly hypothesize a urinary acidification deficit, which was subsequently treated with potassium citrate supplements.</p>
Biochimica Clinica ; 41(2) e13-e14
Casi clinici - Case report
Esame fisico, chimico e morfologico delle urine: proposta di linee guida per la fase analitica del Gruppo Intersocietario Analisi delle Urine (GIAU)
Physical, chemical and morphological urine examination: proposed guidelines for the analytical phase by the Intersociety Urinalysis Group
<p>With these guidelines, the Intersociety Urinalysis Group (GIAU) aims to stimulate the following aspects: a) improvement and standardization of the analytical approach to physical, chemical and morphological urine examination (ECMU); b) to emphasize the value added to ECMU by automated analyzers for the study of the morphology of the corpuscular fraction urine; c) improvement of the chemical analysis of urine with particular regard to the reconsideration of the diagnostic significance of parameters that are traditionally evaluated in dipstick analysis, together with an increasing awareness of the limits of sensitivity and specificity of this analytical method; d) to increase the awareness of the importance of professional skills in the field of urinary morphology and of the relationships with clinicians; e) implementation of a policy for the evaluation of the analytical quality by using, in addition to traditional IQC and EQA, a program for the evaluation of morphological competence; f) to stimulate the diagnostic industry to focus research efforts and development methodology and instrumental catering to the needs of clinical diagnosis. The hope is to revalue the enormous diagnostic potential of ECMU, by implementing an urinalysis based on personalized diagnostic needs.</p>
Biochimica Clinica ; 40(4) 353-382
Documenti SIBioC - SIBioC Documents
Ruolo della spettroscopia infrarossa in trasformata di Fourier nella valutazione della calcolosi urinaria
Role of Fourier transform infrared (FT-IR) spectroscopy in the evaluation of urinary stone disease
<p>Nephrolithiasis is a common disease occurring in both industrialized and developing countries and mainly affecting&nbsp;adults aged 20-60 years. The determination of crystalline and molecular composition and the quantification of all&nbsp;stone components are helpful to establish the etiology of urinary stone disease. Different methodologies exist for the&nbsp;analysis of renal stones. In our laboratory, we used the chemical spot test, which is the most popular method for&nbsp;routine stone analysis. However, this method is relatively inaccurate and does not allow distinguishing between the&nbsp;crystalline phases. In this paper, we evaluated the quantitative FT-IR spectroscopy technique for a possible&nbsp;introduction in our laboratory. We analyzed 100 urinary stones, divided into 3 groups: pure stones, mixed stones, and&nbsp;pure stones with substances in trace. Results of each group were compared with those obtained with chemical spot&nbsp;test. A total or partial disagreement between the two methods was observed in 59% of pure stones, in 86.6% of mixed&nbsp;stones and in 100% of pure stones with substances in trace, respectively, suggesting the replacement of the chemical&nbsp;test with FT-IR spectroscopy.</p>
Biochimica Clinica ; 40(1) 28-32
Contributi scientifici - Scientific Papers