Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
☩Howard Morris Australia
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
☩Jill Tate Australia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Arianna Lucini Paioni
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282
email: biochimica.clinica@sibioc.it

Area soci
Non possiedi o non ricordi la password!
Clicca qui

BC: Articoli scritti da D. Dama

Valutazione preliminare del sistema Alere NS-Plus per la determinazione della transferrina fecale
Preliminary evaluation of the Alere NS-Plus system for the determination of faecal transferrin
L. Bressan  |  N. Corcione  |  D. Dama  |  P. Luraschi  | 
<p>Transferrin (Tf)&nbsp;appears in stools after gastro-intestinal tract bleeding. Compared to hemoglobin, Tf is more stable and resistant to&nbsp;degradation; consequently, its determination in stools has been proposed because it could reveal bleeding that may&nbsp;skip with the hemoglobin research. In this study, faecal Tf was determined with the Otsuka Electronics NS-Plus&nbsp;analytical system, using reagents, control materials and disposable devices from Alfresa Pharma (distributed by&nbsp;Alere). Samples were collected by using a disposable device that allows the draw of a standardized amount of faeces&nbsp;and its dilution in a predetermined volume of buffer.&nbsp;<span style="font-size:10pt">Tf measurements were performed immunochemically with a&nbsp;method based on colloidal gold agglutination. We evaluated imprecision (within-run and total CV) at different&nbsp;concentration levels, linearity, method alignment and limit of blank (LOB). The within-run CV was between 0.9% and&nbsp;23.6% for Tf concentration of 209 and 6 &mu;g/L, respectively. The total CV was 7.4 % and 6.4% for concentrations of&nbsp;46 and 154 &mu;g/L, respectively. The method showed good linearity and agreement between expected and found&nbsp;values (r=0.999) for a concentration interval 5&divide;500 &mu;g/L. The value of the LOB was 1.52 &mu;g/L. In conclusion, in this&nbsp;preliminary evaluation the NS-Plus system showed good analytical performance, possibly permitting its use in the&nbsp;clinical setting.</span></p>
Biochimica Clinica ; 37(4) 279-282
Contributi Scientifici - Scientific Papers