Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali
International Advisory Board
Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
☩Howard Morris Australia
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
☩Jill Tate Australia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada
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Responsible Editor Giuseppe Agosta
Editorial Secretary Arianna Lucini Paioni Biomedia srl Via L. Temolo 4, 20126 Milano Tel. 0245498282 email: biochimica.clinica@sibioc.it
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BC: Articoli scritti da R. Ciuti
Stabilità delle principali droghe d’abuso in campioni di urine non trattate rispetto a campioni di urine stabilizzate
Drug of abuse stability in native urine specimens vs. stabilized urine samples
<p>Effective urine drug of abuse testing requires an understanding of the stability of drugs and their metabolites excreted in the urine matrix. As timing of specimen collection is crucial in urine screening of drugs, potential degradation that may occur between, e.g., the initial test and the re-test of samples, should be considered. Stability characteristics depend on the drug, specimen’s pH, storage temperature, light exposure, bacteria contamination and material of the urine container. The purpose of this study was to determine the stability in urine specimens of four classes of drugs and their metabolites (cannabinoids, opiates, cocaine and methadone) for a maximum of 20 weeks. In the study design, a freshly authentic pooled urine specimen was aliquoted into vials (plastic and glass) and these were stored at freezing (-20 °C), at fridge (4 °C) and at room temperatures and reanalyzed every one/two weeks up to 20 weeks. In parallel, we extended the study analysing one urine pool stabilized using various techniques of thawing and filtration for preventing the decomposition of drugs of abuse. In the native specimens, analytes were relatively stable when stored for extended periods of time at -20 °C. Concentrations of cannabinoids, cocaine metabolites and morphine in not-pretreated samples decreased over time at 4 °C and at room temperature. Methadone did not show relevant loss of drug concentrations during fridge storage. Manufactured urine pool was stable, showing little changes between day 1 and after 10 weeks at 4 °C. Morphine, cannabinoids and methadone showed no loss in drug concentrations after two weeks at room temperature, while benzoylecgonine concentrations significantly decreased after one week.</p>
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