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Maria Stella Graziani

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Martina Zaninotto

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Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
☩Howard Morris Australia
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
☩Jill Tate Australia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada


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Giuseppe Agosta

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Arianna Lucini Paioni
Biomedia srl
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email: biochimica.clinica@sibioc.it



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BC: Articoli scritti da E. Ciusani

La diagnosi di liquorrea nasale e post-chirurgica
Diagnosis of cerebrospinal fluid (CSF) rhinorrhoea and of post-surgical CSF leakage
<p>Pathological CSF leakage&nbsp;outside central nervous system is a very dangerous situation with high risk of meningitis and cerebral abscess.&nbsp;Rhinorrhoea and post-surgical CSF leakage are the most frequent conditions. Diagnosis is made by combination of&nbsp;imaging procedures, radionucleide cisternography via lumbar puncture (fluorescein) and biochemical tests, which utilize&nbsp;markers suggesting the presence of CSF in suspected fluid. Low CSF glucose concentrations suggest rhinorrhoea, as&nbsp;glucose is absent in nose secretion, but cannot be used in post-surgical leakage, as blood is nearly almost present. CSF&nbsp;proteins are the best biomarkers and intrathecal synthesized <span style="font-family:symbol">b</span>-trace protein and transferrin are the best choice. For&nbsp;quantitative analysis, nephelometric <span style="font-family:symbol">b</span>-trace protein measurement has the best performance, as it can be easily&nbsp;automatically performed, also for stat analysis, but relatively high volume of sample is needed. Isoelectricfocusing or high&nbsp;resolution electrophoresis followed by immunodetection are the most sensitive and specific methods for detecting&nbsp;asialotransferrin, but they are time consuming, unsuitable for stat analysis, even if they need low amounts of sample.&nbsp;Other quantitative tests include prealbumin/albumin ratio, having insufficient sensitivity in blood contaminated samples,&nbsp;and zone electrophoresis of protein pattern that, however, has too low sensitivity. New methods like capillary&nbsp;electrophoresis have been recently proposed.</p>
Biochimica Clinica ; 38(3) 183-190
Rassegne - Reviews
 
L’analisi del liquido cefalorachidiano
Cerebrospinal fluid (CSF) analysis
<p>The laboratory investigation of CSF has been developed over the years as a&nbsp;diagnostic tool for many neurological diseases. Although minimally invasive, CSF is obtained with a traumatic&nbsp;procedure; therefore, the whole laboratory process should be established to maximize the analytical performance.&nbsp;Based on the review of international guidelines and on the experience developed by members of the SIBioC Working&nbsp;Group, the present document provides practical information for laboratory professionals to better address the CSF&nbsp;analysis in different diagnostic situations. The report faces the pathophysiologic meaning of the determination of&nbsp;biochemical parameters, such as glucose, lactate, albumin, immunoglobulins, <span style="font-family:symbol">b</span>-amyloid, tau protein, and the cellular&nbsp;content, providing also evidence on the proper methodological approach. Quantitative and qualitative CSF parameters&nbsp;useful to diagnose an inflammatory process of the central nervous system are discussed, particularly with reference to&nbsp;multiple sclerosis. Indications on how laboratory data should be presented to meet international recommendations are&nbsp;also included.</p>
Biochimica Clinica ; 38(3) 238-254
Documenti - Documents
 
L’evoluzione dei criteri diagnostici per la sclerosi multipla non ha modificato la quota di pazienti con bande oligoclonali nel liquor
The evolution of diagnostic criteria for multiple sclerosis (MS) did not change the rate of patients displaying oligoclonal bands in the cerebrospinal fluid (CSF)
<p>The early diagnosis of MS, an inflammatory disease of the&nbsp;central nervous system, can be difficult. Although a specific laboratory test is lacking, the presence of CSF-restricted&nbsp;oligoclonal bands has been reported in the majority of patients (80-95%); as a consequence, this test has been&nbsp;included in the diagnostic criteria for many years. However, after each revision of the diagnostic criteria, the relevance&nbsp;of this test has progressively lost in importance, while magnetic resonance imaging has gained a central role. In the&nbsp;present study, two groups of MS patients diagnosed with McDonald (2001 and subsequent revisions) or with Poser&nbsp;(1983) criteria (176 and 82, respectively) were compared with regard to the frequency of the presence of oligoclonal&nbsp;bands in CSF. The same method for the oligoclonal pattern detection, based on isoelectric focusing followed by&nbsp;blotting and immunodetection of IgG, was used on both groups. Results showed that the rate of patients displaying&nbsp;CSF-restricted oligoclonal bands was 89.0% and 89.8% in the two groups, respectively (P=0.57). Our data suggest&nbsp;that the percentage of MS patients with CSF-restricted oligoclonal bands was unchanged and independent of the&nbsp;diagnostic criteria. Furthermore, the results suggest that the different MS diagnostic criteria considered (Poser vs.&nbsp;McDonald) identify patients with a similar immunological background.</p>
Biochimica Clinica ; 38(3) 213-217
Contributi scientifici - Scientific Papers